The deep sea space station is faced with safety risks in severe weather and low efficiency of underwater multi-platform operation when accompanied by the mother ship for support. The traditional single support ship mode only relies on the underwater positioning methods such as ultra-short baseline,etc. The slow positioning speed,big error and difficult mutual awareness and collaboration of the underwater platform can no longer meet the requirements. A deep sea underwater acoustic positioning method based on communication beacon is proposed in this paper. The time delay estimation is carried out by using the broadband spread spectrum communication,and then the equivalent sound velocity is found by using the established equivalent sound velocity table to correct the sound velocity. In this way,the deep sea underwater acoustic positioning accuracy is improved. Besides,the simulation of estimation target motion trajectory in deep sea hydrological conditions is conducted,and the simulation results show that this method can effectively improve the underwater acoustic positioning accuracy.